Development of regenerating electrochemical biosensors based on aptamer-activated carbon nanotubes and graphene




The research and development of technological bases of formation of electrochemical biological sensors based on carbon nanotubes or graphene modified with specific aptamers to detect the content of viruses, and other disease markers in the blood, facilitating the transition to the establishment of new types of high-speed and selective diagnostic systems.


Carbon nanomaterials, in particular, nanotubes and graphene, are very promising materials to construct biological sensors due to their biocompatibility, high sensitivity to changes in the environment, and simplicity of making sensors based on them. Percolated carbon nanotube networks combine the possibility of simple assembly, processing, and affinity for organic materials with that of the use of a number advantages of inorganic materials in sensing applications. One of the prospects of applying carbon nanomaterials is creating sensor systems using flexible substrates. They make it possible to integrate sensor structures in completely new fields of biomedicine, such as smart skin or wearable monitoring systems.

A sufficiently simple method is suggested to create sensing structures based on the SWNT for biological applications. The sensing structures are formed from the SWNT films on the surface of the flexible polyethylene naphthalate substrate, with the resistance varying within 8–10% and the radius of curvature reaching 5 mm. For the first time, the possibility of creating a selective sensor to thrombin was demonstrated with the additional functionalization of the surface of the SWNT bundles on light, transparent, plastic substrates. The time of response of such a sensor does not exceed 50 s. The increase in the sensitivity and specificity of the biosensors formed to the given proteins might be performed using the corresponding treatment and optimization of the constructional basis of creation of sensing structures.







Main publications

  1. I. I. Bobrinetskii, I. A. Komarov, K. K. Lavrent’ev, D. D. Levin, M. M. Simunin, V. K. Nevolin, L. D. Kvacheva, S. P. Chervonobrodov, A. Burian, L. Khavelek, N. Voznitsa. Features of the Integration of Graphenes in Microelectronic Technology // Russian Microelectronics, 2014, Vol. 43, No. 7, pp. 477–482.
  2. Ahmadishina K.F., Bobrinetskiy I.I.,  Komarov I.A., Malovichko A.M., Nevolin V.K., Petuhov V.A., Golovin A.V., Zalevskiy A.C. Flexible biological sensors based on carbon nanotubes films // Nanotechnologies in Russia, 2013, Volume 8, Numbers 11-12. P. 721-726.
  3. Bobrinetskiy I.I., Seleznev A.S., Gayduchenko I.F., Fedorov G.E., Domantovskiy A.G., Presnyakov M.Yu., Podchernyaeva R.Y., Mikchailova G.R., Suetina I.A. The Interaction Between Nerve Cells and Carbon Nanotube Networks Made by CVD Process Investigation // Biophysics, 2013, Vol. 58, No. 3, pp. 409–414.
  4. Kireev D., Bobrinetskiy I., Seleznev A., Fedorov I., Romashkin A., Morozov R. Transparent and Biocompatible Electrodes Based on Carbon Nanotubes/Albumin Composite // Open Journal of Composite Materials, Vol. 3 No. 2A, 2013, pp. 33-39.




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